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For Saccharomyces cerevisiaea series of plasmids has been developed for the expression of N-terminal and C-terminal in-frame fusions with the protein of interest 45 The dog should be trained to lie on the back seat of the vehicle, clipped to the safety belt.

GFP variants for multispectral imaging of living cells. Interestingly, dog owners will walk with their dog regularly, yet will not walk by themselves.

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